Solid-Phase Radioimmunoassay of Human Growth Hormone
نویسندگان
چکیده
منابع مشابه
Routine ultramicro-measurement of human growth hormone in plasma by solid-phase radioimmunoassay.
plasma based on the solid-phase radioimmunoassay (RIA) using antibody-coated disposable microtiter trays was described. This method is simple, rapid and inexpensive,and particularly useful for ultramicro-measurement of plasma HGH levels in large numbers of plasma samples. The lower limit of sensitivity for purified HGH was 25pg/ml (P < 0.05). The precision of the measurement of HGH in plasma ha...
متن کامل[Radioimmunoassay for human growth hormone].
Detailedmethodsfor: (1) 1311 iodination of human growth hormone (HGH); (2) purification of labeled HGH (HGH-139); and (3) radioimmunoassay of HGH are given. Bio-GeI filtration is introduced as a rapid and reproducible method for purifying HGH-1311which hasbeenobtainedby a modificationof the methodof Greenwood at al. (1). Highlypurified HGH-’311 with a bindability of 96% or more is usually achie...
متن کاملSolid-phase radioimmunoassay of human complement fragment C5a.
In this competitive RIA for determining concentrations of human C5a in biological fluids and in buffers, labeled C5a and sample are allowed to compete for binding to a limited amount of goat antibody to human C5a in solution. Free and bound tracer are then separated by a second antibody (rabbit anti-goat IgG) immobilized on paramagnetic particles. Total incubation time for this assay is 70 min....
متن کامل[Radioimmunoassay of human growth hormone by adsorption to talc].
Radioimmunoassay of human growth hormone (HGH) using adsorption to talc was examined. Results are as follows: 1) The minimal detectable amount of HGH was 0.02 ng/ml. The mean recovery rate was 100.8%. Mean +standard error (S.E.) of the same two samples, repeatedly measured eight times, were 3.5+0.2 and 35.4+0.5 ng/ml, respectively . Concentrations of the same acromegalic plasma diluted in diffe...
متن کاملDevelopment of an in-house radioimmunoassay for human growth hormone.
A simple and sensitive double-antibody radioimmunoassay for human growth hormone (HGH) was developed, optimised and validated. The anti-hGH sera raised in 2 rabbits were highly specific with low cross-reactions of 0.19% and 0.3% with human placental lactogen and 0.21% and 0.13% with human prolactin. The mean sensitivity of the assay determined from 28 assays was found to be 0.4 +/- 0.2 mIU/L. M...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1966
ISSN: 0006-2936
DOI: 10.1042/bj1000031c